Plasmid_Backbone

Part:BBa_K797013

Designed by: Tomohiro Nobeyama   Group: iGEM12_Kyoto   (2012-09-21)

Plasmid Backbone for golden gate assembly

This backbone is suitable for golden gate assembly. Golden gate assembly is a suitable assembly to combine many parts in order you want. This plasmid backbone have restriction enzyme cutting site　of Dpn1 let you build construction by Golden gate assembly in one ligation.

GGA 004.jpg


We conducted Golden gate assembly by ourselves and confirmed this part has the restriction cite and　restriction enzyme cutting site　of Bsa1. After this, we mixed Dpn1 with enzyme-treated plasmid and conducted ligation.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 2197
    Illegal suffix found at 15
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2197
    Illegal SpeI site found at 16
    Illegal PstI site found at 30
    Illegal NotI site found at 23
    Illegal NotI site found at 2203
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2197
    Illegal XhoI site found at 1047
    Illegal XhoI site found at 2073
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2197
    Illegal suffix found at 16
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2197
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2212
    Illegal SpeI site found at 16
    Illegal PstI site found at 30
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 4
    Illegal BsaI.rc site found at 2224


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